© Gunnar Wrobel & Felix Kokocinski,
DKFZ
, 2003 - 2012
Basic parameters
eg APOM
eg ENSG00000111964
eg ENSMUST00000025846
Sus scrofa_wuzhishan
Mus pahari
Varanus komodoensis
Calidris pygmaea
Erinaceus europaeus
Chelonoidis abingdonii
Sus scrofa_bamei
Gadus morhua
Capra hircus
Tursiops truncatus
Sphaeramia orbicularis
Ictalurus punctatus
Mus musculus
Monodelphis domestica
Cyprinodon variegatus
Caenorhabditis elegans
Pseudonaja textilis
Mandrillus leucophaeus
Acanthochromis polyacanthus
Neogobius melanostomus
Esox lucius
Canis lupus_dingo
Peromyscus maniculatus_bairdii
Numida meleagris
Ficedula albicollis
Melopsittacus undulatus
Larimichthys crocea
Dipodomys ordii
Salmo salar
Sus scrofa_hampshire
Pongo abelii
Mus spicilegus
Gouania willdenowi
Cricetulus griseus_picr
Vombatus ursinus
Sinocyclocheilus rhinocerous
Xenopus tropicalis
Serinus canaria
Aotus nancymaae
Mus musculus_cbaj
Cavia porcellus
Poecilia mexicana
Callorhinchus milii
Ochotona princeps
Scophthalmus maximus
Amphiprion ocellaris
Sparus aurata
Seriola lalandi_dorsalis
Cynoglossus semilaevis
Pan troglodytes
Sus scrofa_meishan
Eptatretus burgeri
Periophthalmus magnuspinnatus
Parambassis ranga
Neovison vison
Danio rerio
Gallus gallus
Apteryx haastii
Ciona savignyi
Neolamprologus brichardi
Theropithecus gelada
Oryzias melastigma
Myotis lucifugus
Struthio camelus_australis
Sinocyclocheilus anshuiensis
Nothoprocta perdicaria
Panthera pardus
Meriones unguiculatus
Haplochromis burtoni
Apteryx owenii
Betta splendens
Lates calcarifer
Mus musculus_wsbeij
Heterocephalus glaber_female
Sus scrofa_usmarc
Cricetulus griseus_crigri
Stegastes partitus
Myripristis murdjan
Papio anubis
Macaca fascicularis
Sus scrofa_berkshire
Gambusia affinis
Clupea harengus
Bos mutus
Xiphophorus maculatus
Ursus maritimus
Equus asinus_asinus
Macaca nemestrina
Latimeria chalumnae
Bos indicus_hybrid
Mus spretus
Oryctolagus cuniculus
Echinops telfairi
Anser brachyrhynchus
Amphilophus citrinellus
Aquila chrysaetos_chrysaetos
Crocodylus porosus
Nomascus leucogenys
Homo sapiens
Ornithorhynchus anatinus
Chrysolophus pictus
Cercocebus atys
Vicugna pacos
Bos taurus_hybrid
Prolemur simus
Coturnix japonica
Takifugu rubripes
Hippocampus comes
Notechis scutatus
Ailuropoda melanoleuca
Pundamilia nyererei
Paramormyrops kingsleyae
Gopherus agassizii
Athene cunicularia
Oryzias latipes_hsok
Mus musculus_lpj
Drosophila melanogaster
Strigops habroptila
Seriola dumerili
Saccharomyces cerevisiae
Salmo trutta
Taeniopygia guttata
Otolemur garnettii
Anolis carolinensis
Chlorocebus sabaeus
Colobus angolensis_palliatus
Oreochromis aureus
Xiphophorus couchianus
Bos taurus
Parus major
Phascolarctos cinereus
Pteropus vampyrus
Microcebus murinus
Sus scrofa_tibetan
Ovis aries
Carlito syrichta
Astatotilapia calliptera
Mus musculus_akrj
Fukomys damarensis
Sus scrofa_pietrain
Denticeps clupeoides
Pelodiscus sinensis
Astyanax mexicanus
Bison bison_bison
Sphenodon punctatus
Lepidothrix coronata
Sus scrofa_largewhite
Mus musculus_aj
Cyprinus carpio_huanghe
Salarias fasciatus
Ursus americanus
Rhinopithecus roxellana
Poecilia reticulata
Dromaius novaehollandiae
Camelus dromedarius
Terrapene carolina_triunguis
Cavia aperea
Astyanax mexicanus_pachon
Lepisosteus oculatus
Canis lupus_familiarisgreatdane
Canis familiaris
Callithrix jacchus
Mustela putorius_furo
Monopterus albus
Spermophilus dauricus
Mus musculus_pwkphj
Nannospalax galili
Mus musculus_nzohlltj
Urocitellus parryii
Apteryx rowi
Bos grunniens
Sus scrofa_jinhua
Petromyzon marinus
Hucho hucho
Pan paniscus
Podarcis muralis
Maylandia zebra
Procavia capensis
Oryzias latipes
Rhinopithecus bieti
Manacus vitellinus
Erythrura gouldiae
Cyprinus carpio_germanmirror
Tupaia belangeri
Saimiri boliviensis_boliviensis
Ciona intestinalis
Fundulus heteroclitus
Poecilia formosa
Salvator merianae
Anabas testudineus
Sorex araneus
Mesocricetus auratus
Sinocyclocheilus grahami
Dasypus novemcinctus
Electrophorus electricus
Amazona collaria
Canis lupus_familiarisbasenji
Cyanistes caeruleus
Mus caroli
Choloepus hoffmanni
Jaculus jaculus
Sus scrofa
Rattus norvegicus
Cottoperca gobio
Microtus ochrogaster
Sarcophilus harrisii
Cyprinus carpio_hebaored
Pogona vitticeps
Junco hyemalis
Mola mola
Marmota marmota_marmota
Erpetoichthys calabaricus
Equus caballus
Piliocolobus tephrosceles
Lynx canadensis
Macaca mulatta
Mus musculus_balbcj
Poecilia latipinna
Mus musculus_nodshiltj
Mastacembelus armatus
Anas platyrhynchos_platyrhynchos
Pavo cristatus
Gasterosteus aculeatus
Gorilla gorilla
Zonotrichia albicollis
Oreochromis niloticus
Suricata suricatta
Chrysemys picta_bellii
Mus musculus_casteij
Castor canadensis
Calidris pugnax
Phasianus colchicus
Tetraodon nigroviridis
Meleagris gallopavo
Panthera tigris_altaica
Mus musculus_fvbnj
Physeter catodon
Anser cygnoides
Ictidomys tridecemlineatus
Scleropages formosus
Octodon degus
Catagonus wagneri
Vulpes vulpes
Echeneis naucrates
Cebus capucinus
Sus scrofa_rongchang
Kryptolebias marmoratus
Chinchilla lanigera
Felis catus
Heterocephalus glaber_male
Moschus moschiferus
Amphiprion percula
Pygocentrus nattereri
Lonchura striata_domestica
Accipiter nisus
Oryzias latipes_hni
Sus scrofa_landrace
Loxodonta africana
Notamacropus eugenii
Propithecus coquereli
Labrus bergylta
Rhinolophus ferrumequinum
mM
nM
°C
nucleotides
HTML
XML
TEXT
Specific AutoPrime parameters
nucleotides
Extended Primer3 settings
nucleotides
nucleotides
°C
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%
nucleotides
nucleotides
nucl.
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Internal oligo options
nM
nucleotides
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nucleotides
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AutoPrime
allows to rapidly design primers for real-time PCR measurement of eukaryotic expression. Such primers are usually selected so that they will amplify cDNA generated from mRNA but will not yield a product on the genomic DNA. This is achieved by taking advantage of the eucaryotic splicing system for mRNA. Either primer pairs are selected so that one of the primers matches the exon-exon border sequence that is not present in the genomic sequence or the pair is designed by placing each primer in a different exon so that a genomic product would include a long intronic sequence.
AutoPrime
provides these capabilities on top of the primer3 primer-design software.
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